4In 2007, Barrangou et al. demonstrated for the first time the function of CRISPR/Cas9 as a defensive mechanism of bacteria against bacteriophage attacks by a technique called “plaquing” which builds upon the technique of “plating” bacteria on agar. Plaques of viruses on agar are areas without growth of bacteria due to viral attacks.
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This fragmentation hurts portability. Code that performs well on one runtime may behave differently (or poorly) on another, even though it's using "standard" APIs. The complexity burden on runtime implementers is substantial, and the subtle behavioral differences create friction for developers trying to write cross-runtime code, particularly those maintaining frameworks that must be able to run efficiently across many runtime environments.
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